Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Tomographic three-dimensional imaging of a biological specimen using wavelength-scanning digital interference holography

Open Access Open Access

Abstract

The principle of wavelength-scanning digital interference holography is applied to three-dimensional imaging of a small biological specimen. The images are reconstructed from a number of holograms digitally recorded while the wavelengths are varied at regular intervals, and the numerical interference of the multiple three-dimensional hologram fields results in tomographic images with narrow axial resolution. An animated three-dimensional model of the object is constructed from the tomographic images.

©2000 Optical Society of America

Full Article  |  PDF Article
More Like This
Wavelength scanning digital interference holography for variable tomographic scanning

Lingfeng Yu and Myung K. Kim
Opt. Express 13(15) 5621-5627 (2005)

Supplementary Material (4)

Media 1: MOV (361 KB)     
Media 2: MOV (314 KB)     
Media 3: MOV (332 KB)     
Media 4: MOV (556 KB)     

Cited By

Optica participates in Crossref's Cited-By Linking service. Citing articles from Optica Publishing Group journals and other participating publishers are listed here.

Alert me when this article is cited.


Figures (6)

Fig. 1.
Fig. 1. The apparatus for digital interference holography. RDL: ring dye laser; F1 and F2: neutral density filters; BX: beam expander and spatial filter; BS1 and BS2: beam splitters; REF: reference beam; OBJ: object beam; S: camera’s focal plane; LO: magnifying lens; C: digital camera; Z1: object to hologram distance
Fig. 2.
Fig. 2. a) Direct camera image of the insect under laser illumination. The eyes, the mouthpiece, and the front two or three legs are visible. b) Numerically reconstructed image from one hologram. c) Image accumulated from the 20 holograms, as described in the text.
Fig. 3.
Fig. 3. Digitally recorded optical fields, showing 1×1 mm details out of 4.8×4.8 mm frames: a) hologram, HH*, b) object, OO*, and c) reference, RR*.
Fig. 4.
Fig. 4. (QuickTime, 504k) The animation shows a z-y cross section of the 3D reconstructed field at x=-1.3 mm, as the twenty 3D arrays are added in digital interference holography.
Fig. 5.
Fig. 5. a) (QuickTime, 504k) x-y cross sections of the accumulated array at various axial distances z. b) (QuickTime, 504k) z-y cross sections of the accumulated array at various x-values starting from left end of the head, x=1.84 mm, to near the middle of the head, x=0.52 mm.
Fig. 6.
Fig. 6. (QuickTime, 756k) An animated 3D reconstruction of the insect’s illuminated surface. (Here the insect is facing upward, the vertical being the z-axis.)

Equations (4)

Equations on this page are rendered with MathJax. Learn more.

E k ( Q ) P d 3 r P A ( P ) exp ( i k r P ) .
E ( Q ) k P d 3 r P A ( P ) exp ( i k r P ) P d 3 r P A ( P ) δ ( r P r Q ) A ( Q ) .
E ( x , y ; z ) = exp [ ik 2 z ( x 2 + y 2 ) ] F { E 0 ( x 0 , y 0 ) S ( x 0 , y 0 ; z ) } [ κ x , κ y ]
S ( x , y ; z ) = ik z exp [ ikz + ik 2 z ( x 2 + y 2 ) ] ,
Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved