Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group
Journal Home About Issues in Progress Current Issue All Issues Feature Issues

Third harmonic generation microscopy

Jeff A. Squier, Michiel Müller, G. J. Brakenhoff, and Kent R. Wilson

Open Access Open Access

Abstract

Third harmonic generation microscopy is used to make dynamical images of living systems for the first time. A 100 fs excitation pulse at 1.2 μm results in a 400 nm signal which is generated directly within the specimen. Chara plant rhizoids have been imaged, showing dynamic plant activity, and non-fading image characteristics even with continuous viewing, indicating prolonged viability under these THG-imaging conditions.

©1998 Optical Society of America

Full Article  |  PDF Article
More Like This
Laser scanning third-harmonic-generation microscopy in biology

D. Yelin and Y. Silberberg
Opt. Express 5(8) 169-175 (1999)

Third-harmonic generation microscopy by use of a compact, femtosecond fiber laser source

Andrew C. Millard, Paul W. Wiseman, David N. Fittinghoff, Kent R. Wilson, Jeffrey A. Squier, and Michiel Müller
Appl. Opt. 38(36) 7393-7397 (1999)

Third-harmonic generation microscopy in highly scattering media

Carlo Mar Blanca and Caesar Saloma
Appl. Opt. 39(28) 5187-5193 (2000)

View More...

Supplementary Material (5)

Media 1: MOV (672 KB)     
Media 2: MOV (1327 KB)     
Media 3: MOV (1054 KB)     
Media 4: MOV (644 KB)     
Media 5: MOV (669 KB)     

Cited By

Optica participates in Crossref's Cited-By Linking service. Citing articles from Optica Publishing Group journals and other participating publishers are listed here.

Alert me when this article is cited.


Figures (8)
Fig. 1.
Fig. 1. Microscope showing the input beam and collection beam paths.

Download Full Size | PDF

Fig. 2.
Fig. 2. Upper graph is a measure of the third order power dependence of the signal. The slope is 2.9. Lower graph is a measure of the axial sectioning through a glass/air interface. The excitation objective was 100x/ 1.25 NA, and the collection objective was a 20x/0.6 NA.

Download Full Size | PDF

Fig. 3.
Fig. 3. THG image series through a 250 μm diameter glass sphere. Note all images are raw data - no image processing has been performed, only an artificial color look up table has been used to assign a value to the image intensity. [Media 1]

Download Full Size | PDF

Fig. 4.
Fig. 4. THG image series of Chara rhizoid. [Media 2]

Download Full Size | PDF

Fig. 5.
Fig. 5. THG image series showing cytoplasmic streaming within the rhizoid. [Media 3]

Download Full Size | PDF

Fig. 6.
Fig. 6. THG image series at root tip showing motion of statoliths. [Media 4]

Download Full Size | PDF

Fig. 7.
Fig. 7. High NA series of rhizoid tip. The sections proceed in increments of 1.5 microns, the final image (4) stopping at the outer cell membrane.

Download Full Size | PDF

Fig. 8.
Fig. 8. THG series showing small organisms rapidly moving across and through the image plane. The sample is a drop of pond water. [Media 5]

Download Full Size | PDF

Select as filters
Select Topics Cancel
Top
       
© Copyright 2024 | Optica Publishing Group. All rights reserved, including rights for text and data mining and training of artificial technologies or similar technologies.
Privacy | Terms of Use