Abstract
The generalized phase contrast (GPC) method has been applied to transform a single TEM00 beam into a manifold of counterpropagating-beam traps capable of real-time interactive manipulation of multiple microparticles in three dimensions (3D). This paper reports on the use of low numerical aperture (NA), non-immersion, objective lenses in an implementation of the GPC-based 3D trapping system. Contrary to high-NA based optical tweezers, the GPC trapping system demonstrated here operates with long working distance (>10 mm), and offers a wider manipulation region and a larger field of view for imaging through each of the two opposing objective lenses. As a consequence of the large working distance, simultaneous monitoring of the trapped particles in a second orthogonal observation plane is demonstrated.
©2005 Optical Society of America
Full Article | PDF ArticleMore Like This
Peter John Rodrigo, Ivan R. Perch-Nielsen, Carlo Amadeo Alonzo, and Jesper Glückstad
Opt. Express 14(26) 13107-13112 (2006)
Peter John Rodrigo, Vincent Ricardo Daria, and Jesper Glückstad
Opt. Lett. 29(19) 2270-2272 (2004)
Ivan R. Perch-Nielsen, Peter John Rodrigo, Carlo Amadeo Alonzo, and Jesper Glückstad
Opt. Express 14(25) 12199-12205 (2006)