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Real-time observation of birefringence by laser-scanning surface plasmon resonance microscope

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Abstract

A laser-scanning surface plasmon resonance microscope was used to observe the birefringence of an anisotropic sample in real-time. Since this system utilizes the resonance of the surface plasmon with incident light, it can measure the absolute value of the sample’s refractive index with high sensitivity. In addition, this system can simultaneously measure the orientation of the birefringence. This system does not need any moving optical components, and it can thus obtain the refractive indices in real-time. Experimental results of observing birefringence changes in real-time (video rate) are demonstrated.

©2005 Optical Society of America

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Figures (6)

Fig. 1.
Fig. 1. Schematic diagram of optical setup of laser-scanning surface plasmon resonance microscope.
Fig. 2.
Fig. 2. Optical arrangement for exciting surface plasmons by use of a high-numerical-aperture objective lens. This figure also depicts the creation of a dark ring due to the absorption of the surface plasmon excitation.
Fig. 3.
Fig. 3. The configuration of the liquid crystal cell.
Fig. 4.
Fig. 4. Experimental results of the observed elliptic dark ring. Liquid crystal (LC) molecules were aligned (a) in the horizontal direction, and (b) in the vertical direction.
Fig. 5.
Fig. 5. Relationship between incident angle and reflectance. (a) Schematic diagram of the numerical simulation model. (b) The results of calculation. Lowest reflectance point corresponds to the surface plasmon’s excitation angle.
Fig. 6.
Fig. 6. (2.5MB Movie) Deformation of the dark ring when external voltage is applied and removed.
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