Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Adaptive Scanning Optical Microscope (ASOM): A multidisciplinary optical microscope design for large field of view and high resolution imaging

Open Access Open Access

Abstract

From micro-assembly to biological observation, the optical microscope remains one of the most important tools for observing below the threshold of the naked human eye. However, in its conventional form, it suffers from a trade-off between resolution and field of view. This paper presents a new optical microscope design that combines a high speed steering mirror, a custom designed scanner lens, a MEMS deformable mirror, and additional imaging optics to enlarge the field of view while preserving resolving power and operating at a high image acquisition rate. We describe the theory of operation and our design methodology, present a preliminary simulated design, and compare to existing technologies. A reduced functionality experimental prototype demonstrates both micro-assembly and biological observation tasks.

©2005 Optical Society of America

Full Article  |  PDF Article
More Like This
Quasi-telecentric optical design of a microscope-compatible OCT scanner

Zhilin Hu and Andrew M. Rollins
Opt. Express 13(17) 6407-6415 (2005)

At-focus scanning ptychography for high resolution imaging with a wide field of view

Bojana Ivanic, Jonathan Barolak, Christopher Car, Cameron Clarke, David Goldberger, Jeremy Goeckeritz, Amanda Meier, and Daniel E. Adams
Opt. Express 32(4) 6555-6566 (2024)

Supplementary Material (4)

Media 1: MOV (2515 KB)     
Media 2: MOV (13582 KB)     
Media 3: MOV (2491 KB)     
Media 4: MOV (13671 KB)     

Cited By

Optica participates in Crossref's Cited-By Linking service. Citing articles from Optica Publishing Group journals and other participating publishers are listed here.

Alert me when this article is cited.


Figures (11)

Fig. 1.
Fig. 1. (a) Optical systems image two point sources in the object plane as two Airy patterns in the image plane. Two pixels are required per Airy core radius to avoid aliasing the image. (b) Black box imaging system. (c) Microscope (left) and lithography lens (right). The lens prescriptions for the microscope objective and lithography lens were obtained from [7] and are not shown to scale.
Fig. 2.
Fig. 2. Conceptual layout of the ASOM
Fig. 3.
Fig. 3. Conjugate image and aperture planes
Fig. 4.
Fig. 4. (a) Shape of image field for a thin lens (b) The curved surface of the retina (image sensor) allows for a very simple lens in the human eye (c) The ASOM scanner lens is simplified by allowing a curved image field
Fig. 5.
Fig. 5. Curved image field of scanner lens assembly
Fig. 6.
Fig. 6. Field curvature of scanner lens assembly and imaging optics
Fig. 7.
Fig. 7. ASOM preliminary design
Fig. 8.
Fig. 8. The 40mm virtual field of view of the ASOM is compared to that offered by a traditional microscope using a 1024×1024 and 4096×4096 camera (all systems operating at 0.21 NA). The 0.38mm size of the ASOM sub-field of view is also shown with a 512× 512 camera, requiring many scan movements to cover the entire 40mm field.
Fig. 9.
Fig. 9. (a) Viewing different field positions (b) Optimal deformable mirror shape for the specific field position (c) Strehl ratio sampled over the selected field of view
Fig. 10.
Fig. 10. (774 KB, 14 MB version) Movie demonstrating microgripper tracking with a 3×3 tile mosaic while simultaneously monitoring 2 fixed objects in the workspace.
Fig. 11.
Fig. 11. (1.13 MB, 14 MB version) Movie showing a 3X3 mosaic of living cells taken with the SOMS.

Tables (3)

Tables Icon

Table 1. Qualitative comparison of ASOM to other technologies

Tables Icon

Table 2. Preliminary ASOM performance specifications

Tables Icon

Table 3. Estimated scan times (sec.) for different camera pixel counts and scan rates

Equations (2)

Equations on this page are rendered with MathJax. Learn more.

r = 0.61 λ NA ,
W o = kr 2 .
Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved