Abstract
We recorded one-photon excited fluorescence (1PEF) and two-photon excited fluorescence (2PEF) spectra of purified keratin from human epidermis, and determined the action cross section of this endogenous chromophore. We used this spectroscopic analysis to analyse multiphoton images of skin biopsies and assign the intrinsic fluorescence signals in the epidermis. We observed a good agreement between in situ and in vitro 2PEF spectra of keratin. This study provides a comprehensive characterization of the 2PEF signal of the keratins from the epidermis, and will be of practical interest for multiphoton imaging of the skin.
©2005 Optical Society of America
Full Article | PDF ArticleCorrections
A.-M. Pena, M. Strupler, T. Boulesteix, G. Godeau, and M.-C. Schanne-Klein, "Spectroscopic analysis of keratin endogenous signal for skin multiphoton microscopy: erratum," Opt. Express 13, 6667-6667 (2005)https://opg.optica.org/oe/abstract.cfm?uri=oe-13-17-6667
More Like This
A.-M. Pena, M. Strupler, T. Boulesteix, G. Godeau, and M.-C. Schanne-Klein
Opt. Express 13(17) 6667-6667 (2005)
Hans Georg Breunig, Hauke Studier, and Karsten König
Opt. Express 18(8) 7857-7871 (2010)
M. Strupler, A.-M. Pena, M. Hernest, P.-L. Tharaux, J.-L. Martin, E. Beaurepaire, and M.-C. Schanne-Klein
Opt. Express 15(7) 4054-4065 (2007)