Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group
Journal Home About Issues in Progress Current Issue All Issues Feature Issues

Multiple UV wavelength excitation and fluorescence of bioaerosols

Vasanthi Sivaprakasam, Alan L. Huston, Cathy Scotto, and Jay D. Eversole

Open Access Open Access

Abstract

A two-wavelength laser-induced fluorescence technique is described for detecting and classifying biological aerosols. Single aerosols, smaller than 10 μm, are interrogated with 266 nm and 355 nm laser pulses separated in time by 400 ns. Fluorescence signals excited by these pulses are detected in three broad spectral bands centered at 350 nm, 450 nm and 550 nm. The results indicate that bacterial spores, vegetative bacterial cells and proteins can be differentiated based on the two wavelength excitation approach. Estimates of the fluorescence cross sections for 16 bioaerosol simulants and interferents are presented.

©2004 Optical Society of America

Full Article  |  PDF Article
More Like This
Fluorescence of bioaerosols: mathematical model including primary fluorescing and absorbing molecules in bacteria

Steven C. Hill, Yong-Le Pan, Chatt Williamson, Joshua L. Santarpia, and Hanna H. Hill
Opt. Express 21(19) 22285-22313 (2013)

Spectrally-resolved fluorescence cross sections of aerosolized biological live agents and simulants using five excitation wavelengths in a BSL-3 laboratory

Yong-Le Pan, Steven C. Hill, Joshua L. Santarpia, Kelly Brinkley, Todd Sickler, Mark Coleman, Chatt Williamson, Kris Gurton, Melvin Felton, Ronald G. Pinnick, Neal Baker, Jonathan Eshbaugh, Jerry Hahn, Emily Smith, Ben Alvarez, Amber Prugh, and Warren Gardner
Opt. Express 22(7) 8165-8189 (2014)

Spectral characterization of biological aerosol particles using two-wavelength excited laser-induced fluorescence and elastic scattering measurements

Vasanthi Sivaprakasam, Horn-Bond Lin, Alan L. Huston, and Jay D. Eversole
Opt. Express 19(7) 6191-6208 (2011)

View More...

Cited By

Optica participates in Crossref's Cited-By Linking service. Citing articles from Optica Publishing Group journals and other participating publishers are listed here.

Alert me when this article is cited.


Figures (4)
Fig. 1.
Fig. 1. Schematic of the experimental apparatus used for multiwavelength fluorescence excitation of bioaerosol particles. AC: aerosol chamber; Nd:YAG 1: laser 1, 5 nsec pulses at 1064 nm and 532 nm; THG: third harmonic generator output at 355 nm; Nd:YAG 2: laser 2, 20 nsec pulses at 1064 nm and 532 nm, FHG: fourth harmonic generator output at 266 nm; BS1 beam splitter, 8% reflection at 355nm; BS2: 8% reflection at 266nm; PD1: 355 nm power monitor photodiode; PD2: 266 nm power monitor photodiode; DCM1: dichroic beam splitter reflects 350 nm; DCM2 dichroic beam splitter reflects 450 nm; DCM3: dichroic beam splitter reflects 810 nm.

Download Full Size | PDF

Fig. 2.
Fig. 2. Plots of the elastic scattered light and fluorescence intensity as a function of particle size for un -doped polystyrene spheres showing a cross-sectional area dependence on particle size.

Download Full Size | PDF

Fig. 3.
Fig. 3. Aerosol scattering and fluorescence data summary for the 16 samples listed in Table 1. The bars represent the average value obtained from 500 sequential records. There are six signals recorded for each part icle: (a) scattered light intensity in arbitrary units; (b) 266 nm-excited 350 nm fluorescence signal; (c) 266 nm-excited 450 nm fluorescence signal; (d) 266 nm-excited 550 nm fluorescence signal; (e) 355 nm-excited 450 nm fluorescence signal; (f) 355 nm-excited 550 nm fluorescence signal. The fluorescence signal intensities are given in terms of the number of detected photons.

Download Full Size | PDF

Fig. 4.
Fig. 4. Scatter plot distribution of the 355 nm-excited fluorescence versus the 266 nm-excited fluorescence for the 16 samples listed in Table 1. The ovals represent the probability that a particle of the indicated type will fall within that range.

Download Full Size | PDF

Tables (2)

Tables Icon

Table 1. List of the simulants and interferents investigated

View Table | View all tables in this article
Tables Icon

Table 2. Aerosol samples investigated and estimated fluorescence cross-sections for 266 nm and 355 nm excitation wavelengths in units of cm2/particle

View Table | View all tables in this article
Select as filters
Select Topics Cancel
Top
       
© Copyright 2024 | Optica Publishing Group. All rights reserved, including rights for text and data mining and training of artificial technologies or similar technologies.
Privacy | Terms of Use