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Multiphoton Intrapulse Interference 8. Coherent control through scattering tissue

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Abstract

We demonstrate experimentally that selective two-photon probe excitation using phase shaped pulses can be achieved even when the laser propagates through scattering tissue. The pre-optimized phase tailored femtosecond pulses were able to identify acidic and basic solutions of a pH sensitive chromophore hidden behind a slab of scattering tissue. This observation has important implications for future applications of coherent control for biomedical imaging and photodynamic therapy.

©2004 Optical Society of America

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Figures (5)

Fig. 1.
Fig. 1. Experimental setup. The shaped laser pulses impinge on the sample from right to left. The sample with or without scattering tissue is scanned in the focal plane of the laser while the two-photon induced fluorescence is detected at each point.
Fig. 2.
Fig. 2. Optimized phase functions and the resulting SHG after they are tested with a thin SHG crystal. The panel on the left shows the fundamental spectrum of the pulse and the three different pulses evaluated, transform limited (TL), optimized for acidic excitation (BPS06) and optimized for basic excitation (BPS10). The panel on the right shows the SHG spectrum obtained when each of the laser pulses goes through a thin SHG crystal.
Fig. 3.
Fig. 3. Fluorescence signal obtained from two capillaries with JPTS in buffered solutions. The signal was obtained after excitation with TL and shaped pulses.
Fig. 4.
Fig. 4. Experimental results obtained with TL (black dots) pulses and difference plot obtained from the shaped laser pulses (red circles). Notice that shaped laser pulses are capable of selective excitation even when the laser transmits through scattering tissue
Fig. 5.
Fig. 5. SHG spectrum for TL and for shaped pulses (BPS06) in the presence and absence of biological tissue (intensity multiplied x20).
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