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Manipulation and arrangement of biological and dielectric particles by a lensed fiber probe

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Abstract

An optical trapping system with a single-mode lensed fiber probe inserted at an angle is built; this system is simpler and more flexible than conventional optical tweezers. Two lasers, with 632.8- and 1550-nm wavelengths, are employed to trap and manipulate yeast cells and polystyrene microspheres. Nine yeast cells are manipulated to form a letter “T.” Finally, the manipulation performed with various inclination angles, particle materials, laser wavelengths, and laser powers is analyzed experimentally.

©2004 Optical Society of America

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Figures (4)

Fig.1 .
Fig.1 . Optical forces acting on a particle
Fig.2 .
Fig.2 . Experimental setup. (a) He-Ne laser is employed. (b) 1550-nm laser is employed.
Fig.3 .
Fig.3 . Yeast cell A is always trapped by the probe, and untrapped yeast B moves with the chamber bottom. (a)–(g): The nanostage moves the sample chamber in sequence in directions -y→+y→-x→+x→-z→+z. (g)–(k): Then the micromanipulator moves the fiber probe in sequence in directions +y→-y→+x→-x (observed with a 100× objective).
Fig.4 .
Fig.4 . Nine yeast cells form a letter “T” (observed with a 40× objective).

Equations (1)

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F x = F ax cos θ F tr sin θ
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