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Preliminary study of detecting neoplastic growths in vivo with real time calibrated autofluorescence imaging

Tao Wu, Jianan Y. Qu, Tak-Hong Cheung, Keith Wing-Kit Lo, and Mei-Yung Yu

Open Access Open Access

Abstract

The goal of this study was to evaluate the capabilities of a calibrated autofluorescence imaging method for detecting neoplastic lesions. An imaging system that records autofluorescence images calibrated by the cross-polarized reflection images from excitation was instrumented for the evaluation. Cervical tissue was selected as the living tissue model. Sixteen human subjects were examined in vivo with the imaging system before routine examination procedures. It was found that calibrated autofluorescence signals from neoplastic lesions were generally lower than signals from normal cervical tissue. Neoplastic lesions can be differentiated from surrounding normal tissue based on the contrast in the calibrated autofluorescence. The effects of the optical properties of tissue on the calibrated fluorescence imaging were investigated.

©2003 Optical Society of America

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Figures (4)
Fig. 1.
Fig. 1. (a) A schematic of the F/R colposcopic imaging system. P1 and P2 are the polarizers with their polarization orientations perpendicular to each other. F is the filter to remove the residual reflection of the excitation light. (b) Picture of actual imaging head.

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Fig. 2.
Fig. 2. Typical results obtained from a subject with low-grade SILs and high-grade SILs. (a)raw fluorescence image; (b) cross-polarized reflection image; (c) F/R ratio image.

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Fig. 3.
Fig. 3. F/R ratio image superimposed with real-time reflection image for guided biopsy. (a) (1.6 MB) Video clip of guided biopsy; (b) F/R ratio image.

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Fig. 4.
Fig. 4. Bar chart illustration of the mean values of the F/R ratio for the examined cervical tissues from 16 subjects.

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Equations (3)

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f ( λ em ) = μ s ex l · R 0 ( λ ex ) ( R 0 ( λ ex ) R 0 ( λ em ) ε ( λ ex ) ε ( λ em ) ) 1 2 ( R ( λ em ) R 0 ( λ em ) + ε ( λ em ) ) · F ( λ em ) R ( λ ex )
f ≈ μ s ε l ( R R 0 + ε ) · F R
f ( λ em ) ≈ μ s ex l ( ε ( λ ex ) ε ( λ em ) ) 1 2 R 0 ( λ ex ) ( R 0 ( λ ex ) R 0 ( λ em ) ) 1 2 ( 1 + ε ( λ em ) ) · F ( λ em ) R ( λ ex )
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